Preliminary Investigation of Bioactive Compounds and Bioautographic Studies of Whole Plant Extract of Euphorbia pulcherrima on Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa

Table 2

Different fractions of the ethyl acetate fraction partitioned on silica gel column.

Serial number	Solvent system	Fractions eluted	Colour	Group
1	n-Hexane	1, 2, 3	Black	A
2	n-Hexane	4, 5, 6, 7, 8, 9, 10	Light green	B
3	Hexane : ethyl acetate (9 : 1)	11, 12, 13, 14, 15, 16, 17, 18, 19, 20	White	C
4	Hexane : ethyl acetate (4 : 1)	21, 22, 23, 24	Yellow	D
5	Hexane : ethyl acetate (4 : 1)	25, 26, 27, 28, 29, 30, 31, 32, 33	Dark brown	E
6	Hexane : ethyl acetate (4 : 1)	34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48	Dark green	F
7	Hexane : ethyl acetate (4 : 1)	49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62	Green	G
8	Hexane : ethyl acetate (7 : 3)	63, 64, 65, 66, 67, 68	Brown	H