Archaea

Archaea / 2002 / Article

Research Article | Open Access

Volume 1 |Article ID 516074 | https://doi.org/10.1155/2002/516074

Greg D. Bell, Dennis W. Grogan, "Loss of genetic accuracy in mutants of the thermoacidophile Sulfolobus acidocaldarius", Archaea, vol. 1, Article ID 516074, 8 pages, 2002. https://doi.org/10.1155/2002/516074

Loss of genetic accuracy in mutants of the thermoacidophile Sulfolobus acidocaldarius

Received24 Jul 2001
Accepted02 Oct 2001

Abstract

To investigate how hyperthermophilic archaea can propagate their genomes accurately, we isolated Sulfolobus acidocaldarius mutants exhibiting abnormally high rates of spontaneous mutation. Our isolation strategy involved enrichment for mutator lineages via alternating selections, followed by screening for the production of spontaneous, 5-fluoro-orotate-resistant mutants in micro-colonies. Several candidates were evaluated and found to have high frequencies of pyrE and pyrF mutation and reversion. Neither an increased efficiency of plating of mutants on selective medium, nor the creation of a genetically unstable pyrE allele, could be implicated as the cause of these high frequencies. The strains had elevated frequencies of other mutations, and exhibited certain phenotypic differences among themselves. A large increase in sensitivity to DNA-damaging agents was not observed, however. These properties generally resemble those of bacterial mutator mutants and suggest loss of functions specific to genetic accuracy.

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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