Extensive Lysine Methylation in Hyperthermophilic Crenarchaea: Potential Implications for Protein Stability and Recombinant Enzymes

Table 1

Methylation sites identified in S. solfataricus RNA polymerase subunits^†.

	Protein	Subunit	Residue	Peptide (methyllysine in bold)
1	Sso0225	RpoA^′	395	KELASTLAPGYIIER
2	Sso0225		659	KEIYNEIDR
3	Sso0227	RpoB^′-C	12	IVEKTLYEMGVVPVEEVIR
4	Sso0227		311	GYKGKEYYR
5	Sso0227		349	FLQEFKELSPEQAKR
6	Sso0071	RpoD	115	DIKSEDPSVVPISGDIPIVLLGTNQK
7	Sso0415	RpoE^′	20	IPPNEFGKPLNEIALNELR
8	Sso0415		131	GIIFGEKSKKVIQKGDKVR
9	Sso0415		133	GIIFGEKSKKVIQKGDKVR
10	Sso0415		171	QPYLGKLEWITQTKK
11	Sso0415		179	LEWITQTKK
12	Sso0751	RpoF	54	CDAESAQKVIEELSNIVSR
13	Sso0751		102	TYTSEDIQKIIDIIR
14	Sso5468	RpoH	30	HEVLNIDEAYKILK
15	Sso5468		68	KSQLYGEVVSYR
16	Sso5577	RpoL	71	DALLKAIENIR
17	Sso5577		88	GMTSHYIDEIKGLTK
18	Sso5865	RpoP	19	TFTDEQLKVLPGVR
19	Sso0396	Rpo13	66	KLFEDNYK
20	Sso0396		98	KAKKAVSKKVKKTKKKEKSVEG
21	Sso0396		100	KAKKAVSKKVKKTKKKEKSVEG

Monomethylated lysines are shown in bold. All lysines are conserved in the S. shibatae RNAP sequence with the exception of the lysine in peptide 19, which is an arginine in S. shibatae Rpo13.
^†Supplementary Table 1 contains more detail and evidence supporting the identification of each modification site.