Mutational and Bioinformatic Analysis of Haloarchaeal Lipobox-Containing Proteins
Figure 3
Putative Tat substrate lipoproteins Hvo_B0139, Hvo_1242, DsbA and arabinanase are translocated independent of TatAo in Hfx. volcanii. Western blot analyses of wild-type (RR) Hvo_B0139, Hvo_1242, and DsbA (Hvo_1245) and arabinanase (Hvo_B0232) expressed in Hfx. volcanii wild-type (WT) or TatAo deletion mutants (∆tatAo). All proteins expressed had C-terminal Myc-tags, and were detected using anti-Myc antibodies. Comparable amounts of protein were loaded in each lane. The migration of molecular weight standards is indicated on the right. Predicted positions of precursor (p) and mature (m) proteins are indicated. For comparisons of the unprocessed and processed substrate migration see Figure 2.
