Different Residues on the Surface of the Methanothermobacter thermautotrophicus MCM Helicase Interact with Single- and Double-Stranded DNA
Figure 2
MCM mutant proteins retain their overall structural integrity. (a) Gel filtration analysis of wild-type and mutant proteins was performed as described in “Section 2”. The elution positions of size markers are shown: Thyroglobulin (Thy, 670,000 Da), ferritin (Fer, 440,000 Da), albumin (Alb, 67,000 Da) and ovalbumin (Ova, 45,000 Da). (b) Circular dichroism measurements were performed as described in “Section 2”.
