Different Residues on the Surface of the Methanothermobacter thermautotrophicus MCM Helicase Interact with Single- and Double-Stranded DNA
Figure 3
Helicase activity by the mutant MCM proteins. Helicase assays of wild-type and mutant MCM proteins were performed as described in “Section 2” with 10 fmol of forked (a) or flat (b) DNA substrates. In (a) the protein concentrations used were 3, 9 and 27 nM (as monomer) while in (b) they were 10, 30, and 90 nM (as monomer). The average results of three independent experiments with standard deviations are shown.
