Different Residues on the Surface of the Methanothermobacter thermautotrophicus MCM Helicase Interact with Single- and Double-Stranded DNA
Figure 4
ss and dsDNA binding by the mutant MCM proteins. Nitrocellulose filter binding assays were performed as described under “Section 2” using 50 fmol of 32P-labled ds (a) or ss (b) DNA (49-mer) in the presence of 10, 30, and 90 nM of proteins (as monomer). (c) ssDNA binding assay contained 25 nM protein (as monomer) in the absence or presence of 1 mM ATP. The average results of three independent experiments with standard deviations are shown.
