Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes

Figure 3

Northern blot analysis of M. mazei ΔsRNA₁₅₄ strain. Total RNA was purified from the respective M. mazei strains (ΔsRNA₁₅₄, Δhpt, and wild type) all grown under nitrogen limiting conditions. 10 𝜇g of each RNA were separated by a denaturing 6% PA gel and subsequently analyzed by Northern blot using a ³²P-ATP-labelled oligonucleotide homologous to sRNA₁₅₄. For each sample, the abundance of 5S rRNA was determined to exclude variations in RNA amounts.