Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes

Figure 4

Growth analysis of ΔsRNA₁₅₄ versus wild type under nitrogen limitation (a) and under nitrogen sufficiency: (b) M. mazei strains were grown in 50 mL liquid minimal medium complemented with 150 mM methanol as carbon source under nitrogen limiting conditions (a) and with 10 mM NH₄⁺ as nitrogen source (b) under a gas atmosphere of N₂/CO₂ (80 : 20). Open circles: wild type; closed squares: M. mazei Δhpt; closed triangles: M. mazei ΔsRNA₁₅₄. Standard deviations of five replicates for each strain are indicated.

(a)

(b)