Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures
Figure 6
Relative activity with WT-Ph1prol and prolidase mutants with OP nerve agent DFP. All prolidase assays contained 50 mM MOPS 200 mM NaCl pH 7.0, 0.2 mM CoCl2, and 3 mM DFP. One hundred percent relative activity corresponds to WT-Ph1prol specific activity for DFP of 4 μmol product formed per minute per milligram at 35°C and 10 μmol of product formed per minute per milligram at 50°C.
