Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td align="left"> Prolidase</td><td align="center">Tm (°C)</td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td align="left">WT-Ph1prol</td><td align="center">114.3 ± 1.1</td></tr><tr><td align="left">A195T/G306S1</td><td align="center">114.4 ± 0.1</td></tr><tr><td align="left">Y301C/K342N</td><td align="center">113.8 ± 0.6</td></tr><tr><td align="left">E127G/E252D</td><td align="center">112.2 ± 0.5</td></tr><tr><td align="left">E36V</td><td align="center">114.9 ± 1.3</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td>Tm is the transition temperatures obtained from the analysis of DSC by using the two-state model. The Tm values are the mean of three independent measurements. 1The Tm value is from two independent measurements. </td></tr></table>

Transition temperature of wild type Pyrococcus horikoshii prolidase, Ph1prol, and prolidase variants at pH 7.0.

Archaea

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Table 3

Table 3: Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures

Table 3 | Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures 