Regulatory Multidimensionality of Gas Vesicle Biogenesis in Halobacterium salinarum NRC-1
Figure 5
Mid-culture response of Halobacterium salinarum NRC-1 to changes in shaking speed. Halobacterium salinarum NRC-1 was grown at 100 rpm as described in Materials and Methods. Growth was tracked using both light scattering at 600 nm (solid lines) and by counting colony forming units using spread plate technique (dashed lines). OD600 is reported against the left -axis in logarithmic scale and CFU is reported against the right -axis in linear scale. Half of the sample set were transferred to 150 rpm at ~150 h. 100 rpm shaken cultures are shown as open circles and 150 rpm shaken cultures are shown as closed circles. Points A, B, C, and D refer to panels in Figure 6 in which the phenotype of the cultures are shown for the selected points. Error bars for CFU counts are included as one standard deviation of 24 of 34 triplicate cultures. Error for time points without error bars were too small to display. Points marked with T indicated the points at which cultures were transferred from the slow shaking incubator to the fast shaking incubator.
