Figure 7: Influence of trace element concentration on the growth curve of Halobacterium salinarum NRC-1. Halobacterium salinarum NRC-1 was grown in CM with 0X, 0.25X, 0.5X, 0.75X, 1.0X and 2.0X standard Fe2+ and Mn2+ concentrations (18 μM and 1.9 μM) in a 96-well plate as described in Materials and Methods. Growth was tracked by OD600 and reported against the y-axis in linear scale to highlight phenotypic differences. OD600 values were converted to a path length of 1 cm to be equivalent with other optical density measurements in this manuscript. Each curve is an average of six replicates.