Sulfolobus Mutants, Generated via PCR Products, Which Lack Putative Enzymes of UV Photoproduct Repair

Figure 3

Spectra of spontaneous pyrE mutations in disruptant and control strains. The sequence is of the S.acidocaldarius pyrE coding region, and markings indicate the spontaneous mutations recovered from (a) CS1 (b) CS3 and (c) DG185. Regions removed in deletion mutations are represented by gray italicized text. Tandem duplications (>3 bp) are indicated by an underline or overline. A “+” or a “Δ” sign above a mononucleotide run denotes a single base insertion or deletion, respectively, of the base below it. Placement of these symbols above mononucleotide runs is arbitrary, as all positions of a run are genetically equivalent. Single base pair insertions not located at mononucleotide runs are shown by a “∧” sign with the insertion above it. Bracketed text indicates mutations occurring in a single mutant.

(a)

(b)

(c)