|
Designation | Genotype | Source or reference |
|
S. acidocaldarius strains | | |
|
DG185 | Wild-type | Obtained as ATCC 33909 |
MR31 | (18-bp internal deletion) | [21] |
LR10 | Saci_1227:: | Pyr+ transformant of MR31 (see text) |
LR12 | Saci_1096:: | Pyr+ transformant of MR31 (see text) |
DG251 | Saci_1427:: | Pyr+ transformant of MR31 (see text) |
DG260 | Saci_1227::, | Spontaneous FOA-resistant LR10 |
| | |
DG262 | Saci_1096::, | Spontaneous FOA-resistant LR12 |
| | |
CS1 | Saci_1227:: | DG260 transformed to |
CS3 | Saci_1096:: | DG262 transformed to |
CS1mE | Saci_1227:: | Spontaneous FOA-resistant CS1 |
| G199 | |
CS1m15 | Saci_1227:: | Spontaneous FOA-resistant CS1 |
| A335T | |
JDS8 | G199 | Spontaneous FOA-resistant DG185 (lab collection) |
JDS28 | A335T | Spontaneous FOA-resistant DG185 (lab collection) |
CS3m65 | Saci_1096:: | Spontaneous FOA-resistant CS3 |
| C78 | |
CS3m78 | Saci_1096:: | Spontaneous FOA-resistant CS3 |
| G203A | |
DGm18 | C78 | Spontaneous FOA-resistant DG185 (lab collection) |
DGm23 | G203A | Spontaneous FOA-resistant DG185 (lab collection) |
|
E. coli plasmids | | |
|
pMJ03 | SSV1-derived Sulfolobus shuttle vector | [24] |
pPCBE12 | 3′ portion of Saci_1427 in MCS of pUC19 | Phil Clark (unpublished) |
pLK3a | in MCS (XbaI site) of pNEB193 | This work |
pLK4a | in trpC insert of pPCBE12 | This work |
|
PCR primers | | |
|
SsoPEAvrKpnr1 | GCA GCC TAG GTA CCG ATA TGA GAG AGG TTT ATC CAT TGC | |
SsoPEFKpnAvrf1 | GCA CCT AGG ACG TGT CTT AAT CTC ACA AAG CCC TTA T | |
SacPhr::cassf1 | | |
CAACGAGACGAGAAAATGAAGGAAAATGCCTTGAATAAAGGAATTAAATTTACCGCACAGAGCGTAAGGAGAA |
SacPhr::cassr1 | | |
ATCCACTAATTTTGTCGCAAAATACCTCTCTCCCAGTCTCCAGTCGACAATTATAGTCCTGTCGGGTTTCGCCA |
Saci1096 DTf3 | | |
CACTTAGAATTATTCACTGTTGAGAGTAGGTTACGTATCCAAGTCTAGGGGTACCACGTGTCTTAATCTC |
Saci1096 DTr2 | | |
GATTCACCTTAATAAATCCTCTAATCCAGTTTGTTTAACTCCTAATGCAGCTGGCACGACAGGTTTC |
Saci1227f | AAGAGAGACCACCGGTCTGAATGT | |
Saci1227r | CCT TGGTCATGCCTTCGTGCTAAA | |
Saci_1227internalF | CACCATTTTACACTAAAGCAAGAG | |
Saci_1227internalR | CGACCAACATTCTCACTCTTC | |
NXSaci1096f | GAACGCCGGCTCGAGCAAGAGGGTCAAGTCGATAATTGG | |
NXSaci1096r | GAGGCCGGCTCGAGGGGCGTTTGGTATACTGTTCTATC | |
Saci_1096internalF | GAGTGCTTAAAGTCTCTTCCTC | |
Saci_1096internalR | CTTATCCTTCACCTCAAGCATG | |
|