Sulfolobus Mutants, Generated via PCR Products, Which Lack Putative Enzymes of UV Photoproduct Repair

Table 3

Molecular nature of spontaneous mutations in disruptants versus wild type.

	CS1	CS3	DG185
Frequency of mutations^1
BPS	0.25	0.36	0.26
Transitions	0.13	0.21	0.17
Transversions	0.11	0.15	0.09
Frameshift	0.69	0.55	0.62
plus 1	0.44	0.41	0.40
minus 1	0.21	0.14	0.22
plus 2	0.02	0.00	0.00
minus 2	0.02	0.00	0.00
Del/Ins (≥3 bp)	0.07	0.09	0.12
Deletions	0.02	0.03	0.02
Insertions	0.05	0.06	0.10
P values for hypergeometric statistical analyses^2
Criteria
Position of mutations	.94	.10	—
Type of mutations	.88	.45	—

¹Number of mutations identified in each strain: CS1, 61; CS3, 78; DG185, 107.
² Estimated P values from hyper-geometric tests comparing the mutation spectra of CS1 and CS3 to that of wild type using two criteria, that is, position of mutation regardless of type, and types of mutation regardless of position. For the former, each indel was considered to be located at the first nucleotide affected. The latter used the following 13 categories of mutations: eight frameshifts (+/−A, C, G, T), four base-pair substitutions (A:T → T:A; C:G → G:C; A:T ←→ G:C; A:T ←→ C:G), and indels ≥3 bp.