Acetate Activation in <i>Methanosaeta thermophila</i>: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase

<table>SDS-PAGE analysis of purified soluble pyrophosphatase (Mthe_0236) and AMP-dependent ACS (Mthe_1194). Enzymes were purified by Strep-tactin affinity chromatography. M: molecular mass marker (PAGE Ruler prestained protein ladder, Fermentas, St. Leon-Rot, Germany), lane 1: Mthe_0236 0.5 <svg height="12.175" id="M26" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 19.225 12.175" width="19.225" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,0,0,-.017,.062,7.675)"><path d="M538 96q-38 -45 -84 -76.5t-71 -31.5q-44 0 -15 117q12 49 18 89h-2q-99 -128 -163 -178q-36 -28 -70 -28q-26 0 -44 38h-2q-16 -69 -16 -129q0 -43 11 -75.5t24 -42.5l1 -6q-7 -12 -24.5 -23t-37.5 -11q-40 0 -40 76q0 52 35 202l94 407l78 24l5 -5q-32 -122 -72 -310
q-7 -33 0 -55t25 -22q36 0 105.5 75t107.5 145l32 146l75 26h10q-50 -197 -78 -347q-7 -38 6 -38q7 0 31 17t47 39z" id="x1D707"></path></g><g transform="matrix(.017,0,0,-.017,9.497,7.675)"><path d="M546 430q-14 -33 -37.5 -140t-38.5 -209q-29 -195 -156 -289q-29 -21 -70 -37t-78 -16q-62 0 -102.5 37t-40.5 69q0 27 26 48q19 15 25 -3q13 -43 46.5 -69t87.5 -26q55 0 83 21q29 22 50 65t43 140q14 64 29 163h-2q-55 -81 -149 -152q-59 -44 -102 -44q-23 0 -43.5 30
t-20.5 85q0 74 32.5 145.5t84.5 117.5q40 35 100 58.5t117 23.5q21 0 65 -8q30 -6 44 -6zM456 386q-40 16 -81 16q-29 0 -64 -13q-42 -17 -85 -97q-19 -36 -31.5 -86t-12.5 -86q0 -64 30 -64q20 0 52.5 21t60.5 52q74 81 95 123q26 62 36 134z" id="x1D454"></path></g>
</svg>, lane 2: Mthe_1194 1 <i>μ</i>g.</table>

Archaea

fig1

Figure 1

Figure 1: Acetate Activation in <i>Methanosaeta thermophila</i>: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase 

Archaea

Acetate Activation in Methanosaeta thermophila: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase

Figure 1