Research Article
Acetate Activation in Methanosaeta thermophila: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase
Table 1
Gene number, function of corresponding protein and primers used for amplification of genes analyzed by qRT-PCR.
| Function | Gene number | Primer sequence |
| AMP-dependent ACS | mthe_1194 | for CCAGTGGATCATCGAGTA | rev CAGAAATCGAGGTAGTTC | mthe_1195 | for TAAGGAGCTTGCTGAGAA | rev CAGAACTCTATGTAGTGG | mthe_1196 | for TCGAAGGCGTATGCTGAC | rev CGCCTCGTCAGCCTGCTT | mthe_1413 | for CAGGCGCGCTCCGCGAG | rev GGCCTTTATCGGGATAGG |
| ADP-dependent ACS | mthe_0554 | for TATCATTGGGGTTACAAG | rev CAGAGATGGGTATTGATC |
| PPiase | mthe_0236 | for GCCAGCATGTATGAGCTG | rev CATGTGGGTGACTTGAAT |
| GAP-DH | mthe_0701 | for CTATGCCGTTGCTGTGAA | rev TTGGCGGTGCATTTATCT |
| ribosomal protein S3P | mthe_1722 | for GTTCGTCATGATTGGCAC | rev CCCCTTCTGGAGCTTATC |
| intergenic region | Between mthe_1194 | for GCGGTCAACCTATTTTATTT | and mthe_1195 | rev TTACATACCTCCATTCATCT |
| intergenic region | Between mthe_1195 | for AACGTCCGCAATTTTTATTT | and mthe_1196 | rev CTGCCTCCAGCCCATCCCG |
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