Acetate Activation in Methanosaeta thermophila: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase

Table 2

Activity measurement of the acetyl-CoA synthetase via auxiliary enzymes. The decrease of the absorption of NADH was tracked photometrically at 340 nm.

Enzyme	Reaction catalyzed
Acetyl-CoA synthetase	acetate + ATP + CoA ⇌ acetyl-CoA + PPi+ AMP
Myokinase	AMP + ATP ⇌ 2 ADP
Pyruvate kinase	ADP + PEP ⇌ pyruvate + ATP
Lactate dehydrogenase	pyruvate + NADH ⇌ lactate + NAD^+