|
| Primers | Sequences (restriction enzymes that recognize the underlined sites) |
|
| For the construction of pBAD-MA0606 | |
|
| ma0606fw | GTAAAGAATTCAGATATAAGGAAATAGATGTGATGCTTATGATGCTTAT (EcoRI) |
| ma0606rv | GGTATTTCTAGATTGTATCCTTATTTTTCAGTATTCCCTTGCAATCA (XbaI) |
|
| For the construction of pBAD-MA3969 | |
|
| ma3969fw | TTATATAGCTAGCTATTAAAAATAAGGATAATTAATGCAGGTGGAAGCACACCT (NheI) |
| ma3969rv | GAAATGTCGACGATATATCTCCTTTTATTTTTAGCTTTTTATAGCTGATA (SalI) |
|
| For the construction of pBAD-MA0961 | |
|
| ma0961fw | ATAGAATTCAAGAAGATTATAATGTCTGCCGGAATAC (EcoRI) |
| ma0961rv | GATTCTAGATCATACACCGGCAATGAAAG (XbaI) |
|
| For the construction of pBAD-MA3686 | |
|
| ma3686fw | CTATTGAGCTCAAATAAAAGGAGATATATCATGAAATTGACCATCAATA (SacI) |
| ma3686rv | ATATTGGTACCATCTATTTCCTTATATCTTCAACTTATGACCTTTGTGA (KpnI) |
|
| For the construction of pBAD-ALB2 by amplification of ma0961 | |
|
| alb2fw | ACAATCTAGAGTCGAAGGAAGATTATAATGTCTGCCGGAATAC |
| alb2rv | ATGCCTGCAGGTCGACTCATACACCGGCAATGAAAG (SalI) |
|
| For the construction of pBAD-ALB3 by amplification of ma3969 | |
|
| alb3fw | CGGTGTATGAGTCGAAAGGAGTAATTAATGCAGGTGGAAGCACACCT |
| alb3rv | ATGCCTGCAGGTCGACTTAGCTTTTTATAGCTGATA (SalI) |
|
| For the construction of pBAD-ALB4 by amplification of ma3686 | |
|
| alb4fw | AAAAAGCTAAGTCGAAAGGAGATATATCATGAAATTGACCATCAATA |
| alb4rv | ATGCCTGCAGGTCGACTCAACTTATGACCTTTGTGA (SalI) |
|
