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Research Article

Role of Motif III in Catalysis by Acetyl-CoA Synthetase

Table 2

Kinetic parameters for ACS1Mt wild-type and variant enzymes.

Enzyme 𝑘 c a t 𝐾 𝑚 acetate 𝐾 𝑚 ATP 𝐾 𝑚 CoA
(sec−1)(mM)(mM)(mM)

Acs1Mt 66.6 ± 0.9 3.5 ± 0.1 3.3 ± 0.2 0.19 ± 0.003
Tyr498Ala 1.6 ± 0.04 7.5 ± 0.6 1.7 ± 0.3 0.10 ± 0.004
Tyr498PheInactivea
Thr499Ala 0.8 ± 0.01 3.0 ± 0.011.7 ± 0.1 0.24 ± 0.01
Ala500Thr 1.5 ± 0.04 2.1 ± 0.23.6 ± 0.1 0.51 ± 0.04
Gly501Ala 0.3 ± 0.01 1.4 ± 0.11.7 ± 0.07 0.08 ± 0.002
Asp502AlaInactivea
Asp502GluInactivea
Asp502AsnInactivea

aActivity was tested over a wide range of concentrations for each substrate and at several enzyme concentrations, but no activity was observed.

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