Role of Mn2+ and Compatible Solutes in the Radiation Resistance of Thermophilic Bacteria and Archaea
Figure 2
Protection of enzyme activity with compatible solutes under aerobic conditions. In upper panels, the restriction enzyme DdeI was irradiated up to 12 kGy with 25 mM phosphate buffer (PiB) and the addition of 20 mM trehalose and 0.25 mM Mn2+. In lower panels, the enzyme was irradiated up to 10 kGy, with 25 mM PiB combined with 0.25 mM Mn2+, with the addition of 25 mM MG or DIP. Residual restriction enzyme activity was assayed by the digestion of pUC19 plasmid DNA; fragments were analyzed by agarose gel electrophoresis. The first lanes are molecular size ladders.
