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Volume 2012, Article ID 957852, 10 pages
Research Article

Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

1Department of Basic Medical Sciences, University of Bari Aldo Moro, Piazza G. Cesare, 70124 Bari, Italy
2Institute for Microelectronics and Microsystems, IMM-CNR, National Research Council, 73100 Lecce, Italy
3Institute for Microbiology and Archaeal Centre, University of Regensburg, 93053 Regensburg, Germany
4Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Goethe University of Frankfurt, 60438 Frankfurt/Main, Germany
5Institute for Chemical-Physical Processes, IPCF-CNR, National Research Council, 70126 Bari, Italy

Received 16 July 2012; Accepted 27 September 2012

Academic Editor: Yosuke Koga

Copyright © 2012 Simona Lobasso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N-acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.