Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
Figure 5
(a) Comparison of the MALDI-TOF mass spectra of the lipid component in band 7 of P. furiosus (see TLC in Figure 3) and the authentic standard BPG (i.e., diphytanylglycerol analogue of bisphosphatidylglycerol) isolated from Halobacterium salinarum. (b) PSD fragment ion spectrum of the diphytanylglycerol analogue of BPG of P. furiosus. Abbreviations: PA: diphytanylglycerol analogue of phosphatidic acid; PG: diphytanylglycerol analogue of phosphatidylglycerol; PGP: diphytanylglycerol analogue of phosphatidylglycerolphosphate.
