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Research Article

Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

Table 1

Assignments of m/z values detected in the negative ion mode MALDI-TOF mass spectra and PSD analyses of the P. furiosus total lipid extract to various lipid components. Lipids are indicated as abbreviations.
Observed M–H − signals (m/z)Assignments Calculated M–H − (m/z)
1784.6(Hexose-P)2-T1784.3
1741.5N-Acetyl-hexose-hexose-P-Tcyclic 1741.4
1701.9Hexose2-P-T cyclic 1702.4
1543.5BPG plus Na+1542.3
1521.3BPG1520.3
976.2DGD975.8
934.8N-Acetyl-hexose-P-D934.7
922.6uns-N-Acetyl-hexose-P-D922.6
893.8PI 893.7
881.6unsPI881.5
867.2PGP minus H2O868.6
814.4MGD813.7
805.7PG805.7
787.5PG minus H2O787.7
731.8PA731.6
241.3Hexose-P minus H2O242.0
Abbreviations—D: diether or archaeol; T: tetraether or caldarchaeol; Tcyclic: caldarchaeol with cyclopentane rings; P: phosphate group; uns: unsaturated branched-chains, 3 double bonds for chain; N-acetyl-hexose: N-acetylglucosamine; PI: diphytanylglycerol analogue of phosphatidylinositol; BPG: diphytanylglycerol analogue of bisphosphatidylglycerol (or ether lipid cardiolipin); PG: diphytanylglycerol analogue of phosphatidylglycerol; PGP: diphytanylglycerol analogue of phosphatidylglycerol-phosphate; PA: diphytanylglycerol analogue of phosphatidic acid; MGD: monoglycosyl-diphytanylglycerol; DGD: diglycosyl-diphytanylglycerol.