Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis

Figure 1

Alignment of partial amino acid sequences of the enzyme sulfopyruvate decarboxylase from methanoarchaea, from top to bottom: Methanothermobacter thermautotrophicus, Methanospirillum hungatei, Methanosarcina acetivorans, Methanocaldococcus jannaschii, and Methanococcus maripaludis. The arrows signify insertion positions of the Tn5⟨KAN-2-pac⟩ transposon into the gene comE (558 bp) of M. maripaludis. The insertion at 336 bp (112 aa) corresponds to the mutation in strain M. maripaludis S201, and the insertion at 395 bp (131 aa) corresponds to the mutation in strain M. maripaludis S202. Blue boxes containing aligned amino acids correspond to the conserved thiamine pyrophosphate-binding domain. Asterisks, double dots, and single dots denote positions that contain fully conserved amino acid residues, groups of strongly similar residues, and groups of weakly similar residues, respectively.