Archaea / 2013 / Article / Tab 1

Research Article

Molecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic Archaeon Sulfolobus metallicus

Table 1

Oligonucleotides.

NameSequenceDescription

copA_cdegF5′-gatgtagtaatagtaaaaactggagaaataataccngcngaygg CODEHOP-PCR
copA_cdegR5′-tcatcagcaaaattagaagaatctccngtngcdat CODEHOP-PCR
copT_cdegF15′-ctcaaatagaatataaagtattacaaatgttaaaagargaywsnmgCODEHOP-PCR
copT_cdegR15′-ggattaccatttatttcatttccacartartcrcaCODEHOP-PCR
copT_cdegR25′-cttatcatattcataaatcttccatctattaatttrtarcaytcCODEHOP-PCR
copT_degF15′-gartgytayaarctnatDOP-PCR
copT_degR15′-atnagyttrtarcayctDOP-PCR
copM_degF5′-gayccngtntgyggnatggaDOP-PCR
copM_degR5′-ccnggnttyccntacggDOP-PCR
AdaptF25′-cacgcgtcgactagtactagcttGenome Walking
SP1copA1_3′5′-aaggatgagggggaccttatggGenome Walking
SP2copA1_3′5′-ggagataagaaatggggtaaaagagGenome Walking
SP1copA1_5′5′-tgataccatcatggaacctgtcagGenome Walking
SP2copA1_5′5′-tcctccacaatcccatccgctgGenome Walking
SP1copA1_5′_25′-gattgtagctaagttaacctcggcctcgGenome Walking
SP2copA1_5′_25′-cttctcaccctcagtctggttggGenome Walking
SP1copA2_3′5′-gaaagaggaatatatgcaagggtaaacggGenome Walking
SP2copA2_3′5′-gtgttaatgggagagctggagggGenome Walking
SP1copA2_5′5′-cttctctgtggcaacatcataaccagccGenome Walking
SP2copA2_5′5′-acgcatgtggcgcaatgcattccGenome Walking
SP1copT1_5′5′-cattcctcgcaccagcttgcacactctcGenome Walking
SP1copT2_5′5′-cctatgaatactagatcttttccctgaacGenome Walking
SP2copT2_5′5′-aacagcttataacactcgtcactttggcGenome Walking
copM2_RT_F5′-gatgaaaaaagccaatataagacRT-PCR
copA2_Rv5′-gaacactaactaacatcgccRT-PCR
copM1_RT_Fw5′-ctatcgtttttgttccgaagcttgRT-PCR
copA1_Rv5′-cagcagcaagaacagagacgccRT-PCR
*SM16Sf5′-acgctctaaaaaggcgtgggaata RT-qPCR
*SM16Sr5′-ttgagctcggggtctttaagcagtg RT-qPCR
copA1Sm_qRT_F1 5′-gctaaggtaatagagagcgg RT-qPCR
copA1Sm_qRT_R15′-tgaacaggaatggacaggRT-qPCR
copA2Sm_qRT_F 5′-tgtgcttgtctccttagcgt RT-qPCR
copA2Sm_qRT_R 5′-actcttccgtctttcggagt RT-qPCR
copT1Sm_qRT_F 5′-tgtaggagagtgtgcaagct RT-qPCRl
copT1Sm_qRT_R 5′-tcgcaagtgagggttatggtRT-qPCR
copT2Sm_qRT_F 5′-gtgttacggagcttgca RT-qPCR
copT2Sm_qRT_R 5′-acactcgtcactttggc RT-qPCR

All oligonucleotides were synthesized by Invitrogen. *Oligonucleotides designed by Bathe and Norris [39].