Archaea / 2013 / Article / Tab 6

Research Article

PH1: An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2

Table 6

Transfection of haloarchaea by PH1 DNA.

SpeciesaEfficiency of transfection/transformation with
PH1 DNAb (PFU/μg of DNA)pUBP2 DNAb,c (CFU/μg of DNA)

Har. hispanica 5.3 0.5 × 1031.4 ± 0.8 × 104
Har. marismortui 4.5 ± 0.7 × 1031.8 ± 0.1 × 104
“Har. sinaiiensis” 3.2 ± 0.6 × 102
Halobacterium salinarum 5.4 ± 4.2 × 103
Haloferax gibbonsii 2.8 ± 0.8 × 1034.0 ± 2.6 × 103
Haloferax lucentense 5.7 ± 1.1 × 103
Hfx. volcanii 2.1 ± 0.7 × 105
Hrr. lacusprofundi 7.0 ± 2.2 × 1029.5 ± 1.5 × 103
Haloterrigena turkmenica 1.6 ± 1.7 × 1022.4 ± 0.6 × 103
Natrialba asiatica 3.5 ± 3.9 × 1023.1 ± 1.0 × 104

Only those species positive for transfection and/or plasmid transformation are shown.
Rates of transfection by (nonprotease treated) PH1 DNA or transformation by plasmid pUB2 are averages of three independent experiments, each performed in duplicate (± standard deviation).
Transformants were selected on plates with 2, 4, or 6 μg/mL simvastatin, depending on the strain.
— no plaques or colonies observed.