Research Article
Molecular Tools for the Detection of Nitrogen Cycling Archaea
Table 1
Primer sets for the PCR amplification of archaeal genes that encode key enzymes of nitrogen metabolism.
| Process in N cycle | Key enzyme | Target gene | Primer name | Primer sequence | Reference |
| | Ammonia monooxygenase (E.C. 1.14.99.39.) | amoA | arc-Amo-f arc-Amo-r | 5′-STAATGGTCTGGCTTAGACG-3′ 5′-GCGGCCATCCATCTGTATGT-3′ | [18] | | Ferredoxin nitrite reductase (E.C. 1.7.7.1.) | nirA | arc-NirA-f arc-NirA-r | 5′-AAYMTSCCNCGGAAGTKSAA-3′ 5′-AGAACTCCBTRCCSGTRCAS-3′ | This study | | Ammonifying nitrite reductase (E.C. 1.7.1.4.) | nirB | arc-NirB-f arc-NirB-r | 5′-ATGCTGAGCCATTAYATAGC-3′ 5′-CCGTTGTACTCGGCRCAGTC-3′ | This study | | Nitrous oxide reductase (E.C. 1.7.2.4.) | nosZ | arc-Nos-f arc-Nos-r | 5′-TGGGGHGACTMYCAYCAYCC-3′ 5′-AKGTGKCCRRSGTTGTAGTK-3′ | This study | | Nitrogenase reductase (E.C. 1.18.6.1.) | nifH | arc-Nif-f arc-Nif-r | 5′-TAYGGAAARGGNGGNATYGG-3′ 5′-CCNCCRCAGACRACRTCNCC-3′ | This study | | Dissimilatory nitrate reductase (E.C.1.7.99.4.) | napA, narG | arc-Nred-f arc-Nred-r | 5′-CGACTGGTAYKCVGAYCTHC-3′ 5′-GTCRGYGTKRWACCAGTSGK-3′ | This study |
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