Figure 4: Phylogenetic tree showing the relationships of 16S rRNA sequences recovered from Stone’s Pocket over time. Clones recovered multiple times at 100% sequence identity are indicated with the number of times they were identified listed in parentheses. OTUs at various genetic similarities are marked by overlapping bars and are labeled according to the greengenes taxonomy where appropriate. Phyla are labeled at the appropriate branch points; since WI21 is not within an 80% OTU with any known Euryarchaeota, the corresponding branch point is unlabeled. For clarity, only bootstrap values >90% are shown, and some nodes with values <30% were collapsed. The scale bar represents 0.5 changes per nucleotide. A second version of this tree with clone libraries organized by year is depicted in Supplemental Figure 1.