Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic Archaeon Pyrococcus furiosus

Table 1

Kinetic parameters of ACSx-A isoforms with CoA derivatives.

Enzyme	Substrate	𝐾𝑚  (𝜇M)	𝑘cat/𝐾𝑚  (𝜇M^−1·s^−1)
ACS1-A	acetyl-CoA	137±91	0.140
ACS2-A	acetyl-CoA	350±2	0.114
ACS2-A	isovaleryl-CoA	466±119	0.109
ACS2-A	phenylacetyl-CoA	3320±1320	0.150
ACS3-A	acetyl-CoA	474±165	0.012
ACS4-A	acetyl-CoA	242±46	0.119
ACS5-A	acetyl-CoA	620±1.0	0.112
ACS5-A	isobutyryl-CoA	2730±1080	0.083
ACS5-A	isovaleryl-CoA	820±407	0.120