Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic Archaeon Pyrococcus furiosus

Table 2

Nucleotide kinetics of ACS1 isoforms.

Isoform	Nucleotide	𝐾𝑚  (𝜇M)	𝑘cat/𝐾𝑚  (𝜇M^−1·s^−1)
ACS1-A	ATP	132±19	0.14
ACS1-A	GTP	223±58	0.01
ACS1-G	ATP	342±63	0.011
ACS1-G	GTP	87±7	0.144