The Bifunctional Pyruvate Decarboxylase/Pyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis

Table 1

Purification of bifunctional PDC/POR from T. guaymasensis ^a.

Step	Total protein^b (mg)	Total activity^c (units)	Specific activity^d (U mg^−1)	Purification; (fold)	Recovery; (%)
Cell-free extract	1358	3434	2.6	1	100
DEAE Sepharose	322	1748	5.6	2.2	50.9
Hydroxyapatite	84	580	7.5	2.9	16.9
Phenyl Sepharose	17.9	363	20.2±1.8	7.8	10.6

aCFE was prepared from 25 g (wet weight) of T. guaymasensis cells.
^bAs determined using Bradford assay as described in the Materials and Methods.
^cThe POR activity assayed as described in the material and methods.
^dOne unit was defined as one 𝜇mol of pyruvate oxidized min⁻¹.