The Bifunctional Pyruvate Decarboxylase/Pyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis

Table 2

Purification of bifunctional PDC/POR from P. furiosus ^a.

Step	Total protein^b (mg)	Total activity^c (units)	Specific activity^d (U mg^−1)	Purification; (fold)	Recovery; (%)
Cell-free extract	1267	8442	3.2	1	100
DEAE Sepharose	386	2016	5.2	1.7	23.8
Phenyl Sepharose	80	1031	13.1	4.1	12.2
Gel filtration	28.2	495	19.1	6.0	5.8
Hydroxyapatite	21	392	22.3±2.2	7.0	4.6

aCFE was prepared from 20 g (wet weight) of P. furiosus cells.
^bAs determined using Bradford assay as described in the Materials and Methods.
^cPOR activity assayed as described in the Material and Methods.
^dOne unit was defined as one 𝜇mol of pyruvate oxidized min⁻¹.