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Volume 2014, Article ID 483574, 10 pages
Research Article

Comparative Analysis of the Methanogen Diversity in Horse and Pony by Using mcrA Gene and Archaeal 16S rRNA Gene Clone Libraries

1Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-Cho, Tsu, Mie 514-8507, Japan
2Livestock Breeding and Veterinary Department, Yangon Diagnostic Laboratory, Yangon 11121, Myanmar

Received 24 May 2013; Revised 15 October 2013; Accepted 21 October 2013; Published 30 January 2014

Academic Editor: Stefan Spring

Copyright © 2014 Khin-Ohnmar Lwin and Hiroki Matsui. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Comparative analysis of methanogen compositions in the feces of horse and pony was carried out by constructing the α-subunit of methyl coenzyme-M reductase (mcrA) gene and 16S ribosomal RNA gene (16S rRNA) clone libraries. The mcrA clone library analysis indicated that Methanomicrobiales was predominant in both horse and pony. Furthermore, most of the clones of the 16S rRNA gene library showed that Methanomicrobiales was also predominant in horse and pony, but the LIBSHUFF analysis showed that the horse and pony libraries were significantly different (). Most of operational taxonomic units (OTUs) showed low similarity to the identified methanogens in both the mcrA and the 16S rRNA clone libraries. The results suggest that horse and pony harbor unidentified and novel methanogens in their hindgut. The methanogen population was higher in horse than in pony; however, the anaerobic fungal population was similar in horse and pony. The methanogen diversity was different between two breeds of Equus caballus.