An Antimethanogenic Nutritional Intervention in Early Life of Ruminants Modifies Ruminal Colonization by Archaea
Table 2
Effect of bromochloromethane (BCM) treatment of does and kids on relative abundance (% of total sequences) at weaning, 1 month, and 4 months later in the rumen of kids.
BCM treatment
SED
BCM P value
D− k+
D+ k+
D− k−
D+ k−
kid
Doe
k × D
Weaning
No blast hit
0.2
0.1
f_Methanobacteriaceae
0.1
0.1
g_Methanobacterium
0.2
0.1
1.7
0.1
0.221
0.0094
0.001
0.012
g_Methanobrevibacter
82.2
80.8
71.8
63.3
3.140
0.0002
0.079
0.24
g_Methanosphaera
17
18.2
25.9
36.2
3.110
0.0002
0.052
0.13
f_Methanococcaceae
g_Methanococcus
0.2
f_Methanosarcinaceae
0.1
g_Methanimicrococcus
0.2
0.2
0.1
c_Thermoplasmata
f_WCHD3-02
0.1
0.4
0.2
0.1
0.217
0.28
0.23
0.13
Weaning + 1 month
No blast hit
0.2
0.1
f_Methanobacteriaceae
0.1
0.1
0.1
g_Methanobrevibacter
78.0
91.4
94.7
90.4
3.162
0.025
0.44
0.0005
g_Methanosphaera
21.4
8.1
5.1
9.5
3.153
0.032
0.48
0.0005
f_Methanomicrobiaceae
g_Methanoplanus
0.1
0.2
c_Thermoplasmata
f_WCHD3-02
0.1
0.2
0.1
Weaning + 4 months
No blast hit
0.1
0.1
0.1
p_Crenarchaeota
0.2
0.2
p_Crenarchaeota; c_MCG
0.3
0.4
f_Methanobacteriaceae
0.1
0.1
g_Methanobacterium
0.3
0.2
0.1
0.1
0.096
0.12
0.42
0.73
g_Methanobrevibacter
80.5
85.5
83.4
83.7
2.322
0.78
0.35
0.57
g_Methanosphaera
19
13.3
14.2
14.8
2.160
0.72
0.44
0.22
f_Methanomicrobiaceae
g_Methanoplanus
0.1
f_Methanosarcinaceae
g_Methanimicrococcus
0.1
1
0.1
c_Thermoplasmata
f_WCHD3-02
0.2
0.5
0.6
0.4
0.088
0.32
0.69
0.0006
p: phylum, c: class, o: order, f: family, g: genus; D+ k+: treated kids from treated does; D+ k−: untreated kids from treated does; D− k+: treated kids from untreated does; D− k−: untreated kids from untreated does. Effect of BCM (bromochloromethane) treatment on kids (k), Does (D), and D × k interaction (). SED: standard error of difference.
