An Antimethanogenic Nutritional Intervention in Early Life of Ruminants Modifies Ruminal Colonization by Archaea

Table 3

Effect of bromochloromethane (BCM) treatment of does and kids on diversity (Chao; observed species, OS; and Shannon, H) of the rumen archaeal community in does at weaning and kids at weaning, 1 month, and 4 months later.

Indices		D−	D+		SED	BCM P value
						Doe	kid	D × k
OS		99.7	129.3		12.01	0.027
Chao		135.5	182.5		18.62	0.024
H		4.89	5.43		0.141	0.002
	D− k+	D+ k+	D− k−	D+ k−
Weaning
Chao	90.6	127.4	166.6	182.7	7.370	<0.0001	<0.0001	0.050
OS	66.4	104.9	127.1	131.6	6.647	<0.0001	<0.0001	0.001
H	4.61	4.82	5.82	5.85	0.141	0.21	<0.0001	0.34
Weaning + 1 month
Chao	195.5	172.8	183.8	111.9	19.19	0.001	0.011	0.076
OS	144.0	131.6	133.7	72.0	12.84	0.0003	0.0006	0.010
H	6.10	4.55	5.48	5.18	0.219	<0.0001	0.96	0.0003
Weaning + 4 month
Chao	87.9	117.7	191.4	101.8	6.175	0.0115	0.0005	<0.0001
OS	72.8	86.8	117.1	85.1	4.531	0.16	0.0025	0.001
H	4.63	4.64	4.66	4.70	0.126	0.0739	0.0521	0.092

D+ k+: treated kids from treated does; D+ k−: untreated kids from treated does; D− k+: treated kids from untreated does; D− k−: untreated kids from untreated does.
Effect of BCM treatment of doe (D), kid (k), and D × k interaction (𝑛=8).