Archaeal Enzymes and Applications in Industrial Biocatalysts

Table 1

The kinetic parameters of the activity of the VmutPLL enzyme with different lactone substrates. Taken from Kallnik et al., 2014 [45].

Substrate	Structure	Kcat [s^−1]	Km [mM]	Kcat/Km [s^−1M^−1]
-(R)-valerolactone		6.23 ± 0.42	4.56 ± 0.51	1327.1 ± 61.3
-(S)-valerolactone		2.68 ± 0.00	1.95 ± 0.18	1379.95 ± 110.67
-(R)-caprolactone		3.04 ± 0.01	0.55 ± 0.02	5563.72 ± 140.96
-(S)-caprolactone		1.89 ± 0.11	0.75 ± 0.04	2531.22 ± 298.99
Whiskey lactone		4.20 ± 0.06	0.93 ± 0.07	4538.63 ± 402.78
-Butyrolactone		2.79 ± 0.07	11.57 ± 0.58	241.7 ± 11.18
pNP-acetate		1.66 ± 0.34	8.19 ± 1.10	201.74 ± 20.48
Methyl-paraoxon		1.25 ± 0.40	2.79 ± 0.7	442.58 ± 50.14