Sequence, Structure, and Binding Analysis of Cyclodextrinase (TK1770) from T. kodakarensis (KOD1) Using an In Silico Approach

Figure 2

Phylogenetic tree: rooted radial tree of 17 CD hydrolyzing enzymes was constructed using MrBayes with Wag rate matrix (fixed) and visualized using FigTree. The phylogenetic tree obtained displays three distinct clades. All the bacterial enzymes form a single clade (shown in blue), while the branch for archeal enzymes split into two clades (shown in green and red). Depending upon sequence identity and domain arrangement Tk1770_CDase seems to be more closely related to THEGJ MAse, THES4 CDase, THERCLF CDase, PYRFU NPase, THEON CDase, and PYRYC CDase (green).