Assessment of the Carbon Monoxide Metabolism of the Hyperthermophilic Sulfate-Reducing Archaeon Archaeoglobus fulgidus VC-16 by Comparative Transcriptome Analyses

Figure 2

Overview of shift (ΔCt) in real time PCR cycle threshold (Ct) values between control (dsrB/AF0424) and subsequent assayed transcripts. Average ΔCt values are indicated by bars. The data range for nonsignificant shifts in gene expression is transparent and the data range (min-max) is indicated by boxes. A significant shift (ANOVA) was found between thiosulfate grown cultures (black squares) and cultures with sulfate, or no electron acceptor (gray squares) for AF1101 (ΔΔCt −2.22, ) and AF1667 (ΔΔCt 1.97, 0.04). Low expression levels (i.e., high ΔCt values) indicate lower levels for transcripts of cooS during all conditions and cdhB-1 during growth with sulfate or no electron acceptor.