From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase

<div>Schematic diagram of the three-step reaction catalyzed by ATP-dependent DNA ligases. The three-step reaction catalyzed by DNA ligase (Ligase) results in the serial transfer of AMP (adenosine 5′-monophosphate) to an active site lysine (step 1) and then to the 5′-PO<sub>4</sub> end of DNA (step 2). During step 3, the 3′-OH end of a second DNA strand attacks the 5′-PO<sub>4</sub>, to release AMP and generate the ligated DNA product.</div>

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Figure 1

Figure 1: From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase 