Characterization of Fatty Acids in Crenarchaeota by GC-MS and NMR

Figure 5

NMR analysis of fatty acids from cell extracts. 1D ¹H NMR spectra of S. solfataricus cell extracts (top trace) and lipid standard (bottom trace). A general chemical formula of a saturated fatty acid is illustrated above the top NMR spectrum, with four types of hydrogen atoms experiencing different chemical environments labelled as a, b, c, and d. The characteristic chemical shifts of these chemically distinct hydrogens are assigned correspondingly to specific NMR signals in the top trace NMR spectrum.