Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Figure 3

Results of the transformation of J7-2-1 and J7-2-26 with J7-2 genome. The wild type Natrinema sp. J7-2 total genomic DNA was isolated and then transformed into two auxotrophic mutants, J7-2-1 and J7-2-26. The transformation mixture was spread on the MM plates and cultivated at 42°C for 15 days. The detailed transformation process was described in Materials and Methods. (1) J7-2-1 transformed with J7-2 genome. (2) Negative control, J7-2-1 transformed with salt solution without DNA. (3) J7-2-26 transformed with J7-2 genome. (4) Negative control, J7-2-26 transformed with salt solution without DNA.