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Volume 2015, Article ID 483194, 16 pages
Research Article

Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

1State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
2Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052, China
3Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China

Received 19 February 2015; Revised 30 March 2015; Accepted 23 April 2015

Academic Editor: Sonja-Verena Albers

Copyright © 2015 Jie Lv et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure S1: showed the growth of Natrinema sp. J7-2 on MM plate.

Supplementary Figure S2: showed that in the isolation process of mutants, four strains J7-2-1, J7-2-22, J7-2-26 and J7-2-52 which grow on MGM but failed on MM plate were obtained.

Supplementary Figure S3: the auxotroph of the four auxotrophic strains were reconfirmed with the same experimental procedure that they grow on MGM but failed on MM plate.

Supplementary Figure S4: amplification of important genes in the pathways of the amino acid synthesis from the four auxotrophic strains were shown.

Supplementary Figure S5: showed that the molecular characterization of the gene mutation status of the auxotrophic strains.

Supplementary Figure S6: showed the complementation of the mutation genes by auxotrophic strains’ transformation and comparison the genes of these different transformants.

Supplementary Figure S7: amplification of specific fragments in J7-2-1/ pUC19-HM1752-amyH transformants conferring amylase activity were shown.

  1. Supplementary Figures