Research Article
Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2
Table 2
Primers used in this work.
| Primer name | Sequence (5′-3′) | Restriction site |
| KpnI-1752 | TAAGGTACCTCGCTGCGATCTTGCAGGGACT | KpnI | 1752-MfeI | TAACAATTGCGGCGGTCGCGGGCCACTAAA | MfeI | HindIII-1752 | TTAAAGCTTATGACTCACGAAATCGCCGTCATT | HindIII | HindIII-4049 | TTAAAGCTTATGAGCGCACTCGAAA | HindIII | 4049-MfeI | TATCAATTGCCGGTTACGCCACCCCGCG | MfeI | MfeI-amyH | AATCAATTGATCATATATGCCAACTATTTA | MfeI | amyH-PstI | AAACTGCAGTAGTGGAAAGCGAGCCAGC | PstI | leuA-F | ATGGCACGTTATCCGCCATCT | / | leuA-R | TCAGTCGTCGGCCGGCGT | / | leuC-F | ATGAGCGCGGAAACGCTG | / | leuC-R | TCATACCGAGGTCACCTCCT | / | leuD-F | ATGACGGACGAGGTCGAGATT | / | leuD-R | TACTCCGCTTCGGGAATCGC | / | leuB-F | GTGAGTATGACTCACGAAAT | / | leuB-R | TTACAGTCGGTCGATGAT | / | dapA-F | GCGACCCAAGAGACCACA | / | dapA-R | TCATCGCTCATCCTCCG | / | dapF-F | ATGAATATCCCATTTCAGAAGT | / | dapF-R | TCACGGCTCGGCCGGGGATT | / | lysA-F | ATGAACGAAACCCAAACGTT | / | lysA-R | TATCGGTCGTATTCGTTGTC | / | dapD-F | ATGAGCGCACTCGAAACCGA | / | dapD-R | TTACTCCCGCAGCGCGTCCT | / | dapB-F | ATGACGACGCGAATCGGCGTCAC | / | dapB-R | TCATTCGCTGATCACGTCTGCGAA | / | argF-F | ATGACGACCGATTCCGATCC | / | argF-R | TCACTCGAGCACCCAACTCA | / | argE-F | ATGAGTGCAACCATGGACGACA | / | argE-R | TCATTCGTCGTCCTCGCGAA | / | argD-F | ATGAGCGACCTCGATTTCGT | / | argD-R | TCATGCGTTGGAGTCGGT | / | argB-F | GGGCACCCTAACATGACGAC | / | argB-R | TCATTGGGCCACCTCC | / | argC-F | ATGGCGGTCGGGACCGAG | / | argC-R | TTAGGGTGCCCCCACGGG | / | argXW-F | ATGACCGAATGCGTCGAGTG | / | argXW-R | TCAGGCGGTCACCTC | / | argH-F | ATGACCGAGGAGAGCGCTCAC | / | argH-R | TCAGACATAGCCGTTCACCT | / | argG-F | GATATGACCCGCGTGGCACT | / | argG-R | TTACTGGTCGTCTCCCGTCCC | / |
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