Archaea / 2015 / Article / Tab 5

Research Article

Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Table 5

Percentages of spheroplasts formation and spheroplasts regenerated to wt cells.

Percentage of Spheroplasts formationPercentage of spheroplasts regenerated to wt cells
Different regeneration solutions

About 95%H-2 medium18% MGM mediumRegeneration solution
About 90%About 80%About 20%

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