Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon <i>Natrinema</i> sp. J7-2

<table class="table-group" id="tab5"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left" rowspan="2">Percentage of Spheroplasts formation</td><td align="center" colspan="3">Percentage of spheroplasts regenerated to wt cells</td></tr><tr class="thead"><td align="center" colspan="3">Different regeneration solutions</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left" rowspan="2">About 95%</td><td align="center">H-2 medium</td><td align="center">18% MGM medium</td><td align="center">Regeneration solution</td></tr><tr><td align="center">About 90%</td><td align="center">About 80%</td><td align="center">About 20%</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Percentages of spheroplasts formation and spheroplasts regenerated to wt cells.

Archaea

tab5

Table 5

Table 5: Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon <i>Natrinema</i> sp. J7-2 

Table 5 | Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2 