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Volume 2015 (2015), Article ID 646820, 11 pages
Research Article

Archaeal Communities in a Heterogeneous Hypersaline-Alkaline Soil

1Cátedras CONACYT, Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Km. 10.5 Autopista Tlaxcala-Texmelucan, 90120 San Felipe Ixtacuixtla, TLAX, Mexico
2Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360 Mexico City, DF, Mexico

Received 1 February 2015; Accepted 15 April 2015

Academic Editor: Antonio Ventosa

Copyright © 2015 Yendi E. Navarro-Noya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Rarefaction curves were constructed with the observed OTUs to verify the sampling–sequencing effort (Fig. S1). The observed species richness is larger in soil with electrolytic conductivity (EC) 157.2 dS/m, and lower in soil with EC 0.7 dS/m.

Supplementary Figure 2: A phylogenetic tree was constructed with the sequences found in this study so as to have a more detailed phylogenetic placement of the archaeal phylotypes (Fig. S2). In the Crenarchaeota phylum, the mesophilic Crenarchaeota (ammonia oxidation clade) grouped the majority of the OTUs from the 0.7 dS/m soil. A group of abundant OTUs in this soil was closely affiliated with Candidatus Nitrososphaera gargensis recently suggested as the phylum Thaumarchaeota. OTU 144 with a high abundance in the 0.7 dS/m and 9.0 dS/m soils represented a deep branch in the group 1.1b or soil Crenarchaeota.

Some OTUs belonging to Halobacteriales were detected in both the 9.0 and 139.1 dS/m soils. In general, the OTUs that were more abundant in the MEDIUM soil were more closely related with known genera and species, for example, Halosarcina palida, Halorhabdus tiamatea, Natronococcus amylolyticus, while OTUs more abundant in 157.2 dS/m soil clustered in different mostly unknown branches.

  1. Supplementary Materials