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Volume 2015, Article ID 875784, 12 pages
Research Article

De Novo Sequences of Haloquadratum walsbyi from Lake Tyrrell, Australia, Reveal a Variable Genomic Landscape

1Department of Biological Sciences, Dornsife College of Letters, Arts and Sciences, University of Southern California, 3616 Trousdale Parkway, Los Angeles, CA 90089, USA
2Cooperative Institute for Research in Environmental Sciences, CIRES Building, Room 318, University of Colorado Boulder, Boulder, CO 80309, USA
3Department of Environmental Science, Policy and Management, University of California, Berkeley, 54 Mulford Hall, Berkeley, CA 94720, USA
4Research School of Earth Sciences, The Australian National University, Canberra, ACT 0200, Australia
5Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093-0202, USA
6Marine Biology Research Division, Scripps Institution of Oceanography, La Jolla, CA 92093, USA

Received 19 June 2014; Revised 2 September 2014; Accepted 16 September 2014

Academic Editor: Timothy Williams

Copyright © 2015 Benjamin J. Tully et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Supplementary Material: provides increased levels of analysis and discussion in regards to the “Assembly and Binning”, recruitment of the metagenome on to the H. walsbyi genomes and plasmids from different filter fractions, and the whole genome alignment segments presented in the manuscript, including an additional fourth region of alignment spanning 3,033,000-3,387,000 bp along J07HQW1.

Supplementary figures: illustrate the raw results of the tetranucleotide binning protocol and offer more details for the whole genome aligned segments. Supplementary tables include details regarding the Halobacteriaceae genomes used to construct the local database, changes to assembly efficiency through the various round of assembly, the genes identified as variant ABC-transporter subunits, and percent recruitment of the Lake Tyrrell H. walsbyi genomes from different filter fractions of the metagenome.

  1. Supplementary Material