Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus

Figure 3

Biohydrogen production from glucan and water via SyPaB. SHI is colored in red and the model of tetrameric SHI (see Figure 1) shows how NADPH is oxidized to produce hydrogen. The abbreviations are GNP, glucan phosphorylase; PGM, phosphoglucomutase; G6PDH, G-6-P dehydrogenase; 6PGDH, 6-phosphogluconate dehydrogenase; R5PI, phosphoribose isomerase; Ru5PE, ribulose 5-phosphate epimerase; TKL, transketolase; TAL, transaldolase; TPI, triose phosphate isomerase; ALD, aldolase; FBP, fructose-1,6-bisphosphatase; PGI, phosphoglucose isomerase. g1p, glucose-1-phosphate; g6p, glucose-6-phosphate; 6pg, 6-phosphogluconate; ru5p, ribulose-5-phosphate; x5p, xylulose-5-phosphate; r5p, ribose-5-phosphate; s7p, sedoheptulose-7-phosphate; g3p, glyceraldehyde-3-phosphate; e4p, erythrose-4-phosphate; dhap, dihydroxyacetone phosphate; fdp, fructose-1,6-diphosphate; f6p, fructose-6-phosphate; and Pi, inorganic phosphate. Modified from [14].