Research Article

A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans

Table 2

Kinetic parameters of Asac_1390.

Substrate
(mM)

(s−1)
/
(s−1 mM−1)

(U mg−1)

oNPGal2.0434.0217.0468.9
pNPGal2.9461.8159.8499.3
pNPGlu0.24318.51327.1343.9
pNPXyl4.65114.624.6123.5
pNPMan2.458.224.262.8

The reaction was carried out in 100 mM sodium phosphate buffer (pH 7.0) at 93°C. Data represent the means of three separate experiments.